The Life Science X-ray Scattering (LiX) beamline at NSLS-II of Brookhaven National Laboratory is part of the LSBR research resource jointly funded by the NIH and DOE-BER. The LiX beamline supports several modes of x-ray scattering for investigating the structure of biological macromolecules in solution. Most of the experiments are carried out in the high throughput mode, in which data are collected from ~50 microliter of sample in two minutes. Robotic sample handling is currently under development is extend automated data collection to 360 samples, to enable unattended overnight operations. For samples that contain multiple species of particles (e.g. coexisting complex and subunits) or may contain aggregates, the instrument can instantaneously switch to the in-line size exclusion chromatography mode, essentially using the beamline as one of the detectors of a HPLC purification system. We have implemented software to deal with drifting scattering background and overlapping structural species. The instrument is equipped with an x-ray fluorescence detector to facilitate anomalous scattering measurements. We are also exploring time-resolved solution scattering measurements based on flow mixers. In all measurements, the scattering data are recorded on three detectors to cover a wide range of scattering vectors, typically from 0.006 to 3.2 Å<sup>-1</sup>. Access is flexible through regular general user, rapid access, and block allocation group proposals. Once robotic sample handling is fully operational, mail-in and remote access will also be offered.

Biomolecular solution scattering at the life science X-ray scattering beamline

2019 Spring National Meeting

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