Molecular subtyping of breast cancer remains a challenge in clinic medicine, which plays a crucial role in the treatment. In this study, four ssDNA aptamers against MCF-7 breast cancer cells were developed by Cell-SELEX for differentiating breast cancer subtypes. Among all these aptamers, aptamer MF3 showed better specificity than other apatmers as well as favorable binding affinity towards MCF-7 cells with a Kd value of 82.25 ± 25.14 nM. Aptamer MF3 can not only successfully distinguish MCF-7 breast cancer cells from MDA-MB-231, SK-BR-3 breast cancer cells and MCF-10A human normal mammary epithelial cells, but also can differentiate MCF-7 cells from other cancer cells or normal cells, such as A549 lung cancer cells, HepG2 hepatocellular carcinoma cells, L02 normal hepatic cells, HeLa cells, A375 melanoma cells, SGC-7901 gastric carcinoma cells and GES-1 gastric epithelial cells. Moreover, the results of <i>in vivo </i>fluorescence imaging of tumor-bearing mice indicated that aptamer MF3 can distinguish the xenografts of MCF-7 cells tumor-bearing mice from that of MDA-MB-231 and SK-BR-3 cells tumor-bearing mice <i>in vivo</i>. Finally, the bio-distribution of aptamer MF3 also suggested its potential application for the targeted therapy of breast cancer. Collectively, all these results demonstrated aptamer MF3 a promising highly-effective theranostic tool for the personalized medicine of breast cancer.

Specific DNA aptamers selected for molecular classification of breast cancer subtypes

2019 Spring National Meeting

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