In this contribution we will describe an alternative to mass spectrometry-based analysis of glycans by the use of lectins for development of various types of bioanalytical devices. Electrochemical biosensors constructed with interfacial layers controlled at nanoscale can detect glycans down to a single molecule level (i.e. aM level) and some of the approaches developed by us are still the most sensitive for particular analytes. The biosensors were extensively optimized to resist non-specific interactions allowing to work with complex samples such as human serum from patients having various diseases, lysates from isolated human cells and flu viruses. The most recent approach involves use of magnetic nanoparticles for highly sensitive assay format requiring small sample consumption. The potential of lectin-based glycan analysis will be provided by the use of the bioanalytical devices for analysis of serum samples from people having prostate cancer, breast cancer, rheumatoid arthritis and chronic lymphocytic leukaemia. The clinical potential of magnetic enzyme-linked lectin assays for diagnostics of prostate cancer will be discussed. The last part of the presentation will show application of glycan biosensors with an immobilized Tn antigen to detect antibodies. Two different approaches were applied for immobilization of a small glycan either directly to a modified surface (2D biosensor) or to a layer of human serum albumin formed on the surface (3D biosensor) and these two approaches are compared.

Nanotechnology-based glycan analysis: Application in glycomics and diagnostics

2019 Spring National Meeting

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