We design a novel dual-mode optical (i.e., fluorescence and UV-vis absorption) method for sensing of phosphate compounds (PCs) (e.g., pyrophosphate and nucleotides) based on pattern recognition, which employs porphyrin/lanthanide-doped upconversion nanoparticles (Ln-UCNPs) hybrid combined with an ingenious pH-regulated strategy as the sensor array. A readily available porphyrin hydrate (tetraphe-nylporphyrin tetrasulfonic acid hydrate, TPPS) can assemble onto the ligand-free Ln-UCNPs to form the organic/inorganic hybrid (TPPS/Ln-UCNPs), resulting in a new absorption band to quench the upconversion fluorescence of Ln-UCNPs due to fluorescence resonance energy transfer (FRET). The dual-mode optical property of TPPS/Ln-UCNPs are quintessentially associated with the pH in aqueous solution. Therefore, as a proof-of-concept design, we prepared three types of TPPS/Ln-UCNPs (TPPS/Ln-UCNPs4, TPPS/Ln-UCNPs4.5, and TPPS/Ln-UCNPs5) by using buffers with different pH (at 4, 4.5, and 5) to form a facile sensor array, which would result in individual dual-mode optical response patterns upon being challenged with PCs for their pattern recognition via a competitive mechanism between PCs and TPPS. The results show that three TPPS/Ln-UCNPs sensors can sensitively detect 14 PCs and distinguish them between different concentrations, as well as a mixture of them.<br/>

pH-regulated optical performances in organic/inorganic hybrid: A dual-mode sensor array for pattern recognition-based biosensing

2019 Spring National Meeting

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