Membrane fouling commonly occurs in all filter types during virus filtration in protein-based biopharmaceutical manufacturing. Mechanisms of decline in virus filter performance due to membrane fouling were investigated using a cellulose-based virus filter as a model membrane. Filter performance was critically dependent on solution conditions; specifically, ionic strength. To understand the interaction between immunoglobulin G (IgG) and cellulose, sensors coated with cellulose were fabricated for surface plasmon resonance measurement. The primary cause of flux decline appeared to be irreversible IgG adsorption on the surface of the virus filter membrane.  Analyses of adsorption in IgG molecules on cellulose surfaces mimicking cellulose-based virus removal membranes provide an effective approach for identifying ways of optimizing solution conditions to maximize virus filter performance.

Development of a method for the measurement of Interactions between protein molecules and the virus removal membrane surface: effects of immunoglobulin G adsorption on filter performance

2019 Spring National Meeting

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