Catechins are plant-based flavonoids that are credited with an antioxidant activity. In this project, high performance liquid chromatography coupled with photodiode array and mass spectrometry (HPLC-PDA-MS) was utilized for the determination of catechins and caffeine in four green tea extracts. The separation was achieved using an aqueous methanol gradient and a silica-C18 Brownlee Choice column. The mobile phase was enriched with 0.1% acetic acid for an enhanced positive mode electrospray ionization response. The identity of analytes was confirmed by comparing retention times, UV, MS and MS/MS spectra with corresponding standards. All four green tea extracts were determined to contain (-)-gallocatechin, (+)-catechin, (-)-epigallocatechin, caffeine, (-)-epigallocatechin 3-gallate, (-)-epicatechin, and (-)-epicatechin 3-gallate.

Determination of catechins in green tea extracts using high performance liquid chromatography coupled with photodiode array and electrospray ionization mass spectrometry

2019 Spring National Meeting

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