Much time and effort has been focused on engineering nanoparticles to interface with proteins. In contrast, there have been few examples of engineering proteins to interact with nanoparticles. We have fabricated self-assembled superstructures through co-engineering of recombinant proteins and nanoparticles. These structures feature an unprecedented level of multi-layered hierarchical organization of the components: individual proteins and nanoparticles co-assemble to form discrete assemblies that collapse to form granules, which then further self-organize to generate superstructures &gt;100 nm in size. These hierarchical hybrid materials have been generated using a wide range of proteins, providing direct cytosolic delivery of the protein into cells. We have employed this system to co-deliver complete CRISPR machinery (Cas9 protein and guide RNA) for effective editing both in vitro and in vivo.

Nanoparticle-mediated assmbly of engineered proteins: New structures with unique cellular delivery capabilities

2019 Spring National Meeting

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