Cell-free protein synthesis (CFPS) systems have now made point-of-care protein therapeutics a reality<sup>1</sup>. Our miniaturized system for protein production-on-demand (Mini-PPOD) is aimed at being portable, affordable and capable of producing single-dose amounts of biotherapeutics. This system is compatible with any CFPS system and the end-to-end protein production is integrated within a suitcase/valise. This integrated system is assembled on a Labsmith® (Livermore, CA) microfluidic platform and consists of three main components; 1) A mini-bioreactor equipped with a continuous collection channel for harvesting the target protein, 2) a modular microfluidic purification chip for affinity-based protein purification, and 3) process analytical technologies: (PAT) (e.g. pressure, temperature, UV detector, etc.) throughout the production process that provides a real-time process foot-print. For a protein concentration of 50-250 µg/mL of purified green fluorescent protein (GFP) (produced in a Chinese hamster ovary (CHO)-CFPS system), silver staining revealed high purity in the eluted fractions of about 95-98%, with a &lt;40% product loss from harvest. Figure 1, A) is a picture of our Mini-PPOD suitcase kit and B) is the purification process profile for GFP eluted at 30 mins. This portable platform is envisioned as being part of a first responder's medical kit and may offer a therapeutic protein dose at the patient’s bedside.

Honey, I shrunk the biologics manufacturing facility!

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.