2-Nitropropane has been identified by the United States Food and Drug Administration as a harmful or potentially harmful constituent present in cigarette smoke, but few methods exist for the quantitation of this compound in tobacco due to low selectivity and sensitivity.  An isotope dilution gas chromatography-tandem mass spectrometry method has been developed for the quantitation of 2-nitropropane in mainstream cigarette smoke. Sample cleanup and concentration was accomplished using solid phase extraction. The method demonstrates no measurable matrix effects and an adequate sensitivity to measure vapor phase 2-nitropropane concentrations in the nanogram range, with an 18.7 ng/cig method limit of detection. Product variability estimated from the analysis of 15 cigarette products yielded relative standard deviations ranging from 5.4% to 15.7%, and estimates of precision from two quality control products (University of Kentucky 3R4F and CORESTA Monitor #6 test pieces) yielded relative standard deviations of 9.49% and 14.9%. Under the Health Canada Intense smoking regimen, 2-nitropropane in machine-generated mainstream smoke from 15 cigarette products was found to range from 98.3 to 363 ng/cig.<br/>

Gas chromatography-tandem mass spectrometry method for the selective determination of 2-nitropropane in mainstream cigarette smoke

2019 Spring National Meeting

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