Two-dimensional channels were patterned on films prepared from cellulose nanofibrils (CNF) using photolithography and inkjet printing. Such processes included UV-activated thiol-yne click coupling and inkjet-printed designs developed with polystyrene. The microfluidic channels were characterized (SEM, wetting and liquid flow) and applied as platforms for biosensing. Compared to results from the click method, a better fidelity and fluid flow properties were developed with the simpler inkjet-printed channels. Surface Plasmon Resonance (SPR) and AFM imaging were used to reveal antifouling properties and non-specific adsorption. For this, a target protein (human immunoglobulin G, hIgG) was used to test the fluidic systems after surface modification with bovine serum albumin (BSA), fibrinogen and block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) (PDMAEMA-block-POEGMA copolymers). In addition, confocal laser scanning microscopy allowed the identification of diffusion and adsorption traces in the channels. The results confirmed an effective surface passivation of the microfluidic channels (95% reduction of hIgG adsorption and binding). The inexpensive and disposable systems proposed here allow designs with space-resolved blocking efficiency that offer a great potential in biosensing applications.

Two-dimensional antifouling fluidic channels on cellulose nanopaper

2019 Spring National Meeting

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