3D Bioprinting is a revolutionary technology for the design of tissue models with advanced functionality. The use of cell instructive bioinks in 3D Bioprinters allows for the rapid manufacturing of 3D tissues and organs <i>in vitro</i> and the precise control over microarchitecture and functionality of cell-laden constructs. The printing versatility of bioinks together with living cells eliminates the risk for poor cell viability and cell accessibility throughout the 3D network. In the peresent work, we demonstrate a few examples of cell instructive bioinks prepared with chemically modified cellulose nanofibrils (CNFs). The CNFs were bioconjugated with cell adhesive peptides (GRGDSP) and laminin proteins (LN521) in order to promote human dermal fibroblast adhesion and viability in the scaffolds. The degree of bioconjugation, colloidal stability and chemical composition of the modified CNFs were analyzed by chemical characterization methods. The printability of the bioinks was evaluated with a 3D printer under different printing conditions. The influence of peptide/protein modification and the bioink composition on human dermal fibroblast (HDF) adhesion, viability and proliferation were studied <i>in vitro</i>. The results showed that the adhesion, viability and proliferation of the HDFs were promoted significantly in the 3D constructs bioprinted with GRGDSP modified bio-inks. Improved cartilage-like tissue formations were achieved in the LN521-CNF/Alginate bioinks after two weeks of differentiation of human-derived induced pluripotent stem cells (iPSCs).

3D Bioprinting of soft tissues using nanocellulose-based cell instructive bioinks

2019 Spring National Meeting

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