In this work, gold nanoparticle arrays are used for copper detection at different physiological concentrations (40-100 μM). This is accomplished using a combination of click chemistry, localized surface plasmon resonance (LSPR), and molecular electronic resonance. The coupling between the LSPR and molecular electronic transition increases the sensitivity of the gold nanoparticle array, which creates larger optical shifts which is monitored by UV-Vis spectroscopy. The arrays are fabricated using hole mask lithography, followed by a thin silica coating, and the addition of an alkyne. The click chemistry involved is a copper dependent (Cu+) reaction, in the presence of Cu+ ions a molecular resonant dye (5/6-Texas Red-PEG3-Azide) is cyclized and covalently attached to the gold nanoparticles. Since Cu+ is required to link the dye to the nanoparticle surface, higher concentrations of Cu+ result in larger amounts of dye being coupled to the surface, producing larger optical shifts. The data presented compare the optical shifts observed, using UV-Vis spectroscopy, as a function of Cu+ concentration. Our data show that this sensor can be used to detect Cu+ at physiological concentrations.

Sensitive, selective, and quantitative copper sensor using click-chemistry with gold nanoparticles

2019 Spring National Meeting

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