We study membrane dynamics of phospholipid liposomes in aqueous solutions using Fast Field Cycling (FFC) 1H NMR and Field Cycling (FC) 31P NMR. Proton Fast Field Cycling NMR allows us to investigate dynamics over a wide time range spanning from translational diffusion to order fluctuations to individual lipid rotations. We investigate the differences in dynamic processes occurring with changes in environmental conditions. Further, we employ 31P Field Cycling NMR technique which will help understand unique dynamics in the zwitterionic phosphatidylcholine head group. The sensitivity to headgroup motions allows us to simplify the dynamics and obtain a better understanding of the surface dynamics of the bilayer. Finally, we combine the investigated dynamics with the results obtained by Neutron Spin Echo (NSE) in order to obtain a broader picture of the dynamics occurring in phospholipid liposomes and their uniqueness in serving as a model for understanding cell membrane dynamics.

Understanding the dynamics of phospholipid membranes using field cycling NMR

2019 Spring National Meeting

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