Ras and many other proteins that are oncogenic, i.e., associated with cancer when mutated, are allosteric switches. The growing power of gene synthesis and next-generation sequencing can be harnessed to yield insights into molecular mechanism in a novel way. I will cover the application of high-throughput mutagenesis to Ras, combined with structural analysis and molecular dynamics simulations, with an emphasis on understanding the allosteric mechanisms that lead to mutational sensitivity.

Understanding molecular mechanisms in oncogenic proteins through high-throughput mutagenesis: Application to Ras

2019 Spring National Meeting

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