The use of direct oral anticoagulants (DOACs) has been increasing due to convenient dosing and without routine monitoring. However, monitoring of DOACs in certain high risk populations can improve patient managements. These special populations for examples are patients with bleeding complications, surgical patients and patients with thromboembolic events while taking the DOACs. Currently, DOACs can be monitored with coagulation makers, laboratory associated chromatographic instruments, and thrombin generation assay. These methods are either non-specific or require specialized expensive equipment. Therefore, there is a strong need to identify binding elements with high affinity and specificity that can facilitate the direct monitoring of DOACs levels in clinically relevant samples. This study focuses on the identification of a single-stranded DNA(ssDNA) aptamer that binds to an DOAC. In this study we use a variation of the Systematic evolution of ligands by exponential enrichment (SELEX) process to identify an aptamer which binds to a DOAC with high affinity and specificity. The stringency of our method of SELEX and focus on negative selection will allow us to introduce our aptamer candidate into a proof-of-concept biosensing platform.

Identification of ssDNA aptamer specific to an oral anticoagulant

2019 Spring National Meeting

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