Electrogenerated chemiluminescence (ECL) based biosensors combine the high sensitivity of a luminescence biosensor with the low cost and miniaturization capabilities of electrochemistry. A stem-loop DNA probe was immobilized onto a gold electrode via an alkanethiol linkage. The electrodes were then passivated using alkanethiols of different carbon chain lengths (C6, C8 and C9). Upon introduction of single stranded DNA target, a conformational change of the probe led to a change in the ECL intensity. Sensors utilizing C8 passivation chemistry demonstrated both the greatest stability and ECL signal change. These sensors were observed to have signal-off behavior in the presence of 2-μM complementary K-ras DNA target. The sensors did not respond the same when the non-complementary DNA target sequence was added, demonstrating some specificity. This study also compared the response of the sensors in the presence of two different co-reactants , tri-propyl amine and dibutylaminoethanol.

Work towards an electrogenerated chemiluminescence -DNA biosensor labeled with a ruthenium complex

2019 Spring National Meeting

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