Detection of biogenic amines is important due to their link with physiological processes such as food-related illnesses and histamine poisoning. Electrogenerated chemiluminescent (ECL) detection offers an inexpensive and portable method for detecting for biogenic amines when coupled with low-cost screen-printed carbon electrodes and mobile phone camera detectors. In this work, a mobile phone camera was comparatively evaluated against a CCD camera setup for the detection of ECL from the reaction of biogenic amines with a ruthenium complex. A 3-D printed light-tight casing was produced and its improvements on the analytical capacity of a mobile phone camera were quantified. Reaction conditions such as applied potential and pH were optimized and the method was used to detect spermidine, putrescine, and histamine at concentrations below the threshold of human toxicity. This ECL detection method was applied to simulated spoiled milk samples and various methods of sample preparation were evaluated.

Development and evaluation of mobile phone camera as an electrogenerated chemiluminescence detection method for biogenic amines using a ruthenium complex

2019 Spring National Meeting

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