The need for a robust, specific and high-throughput biosensing platform is of significant interest in analysis of glycans. However, current analytical technology requires long run times, sophisticated and expensive equipment. Paper based biosensors provide alternative approaches for rapid and easy-to-use assays. We have developed assays with colorimetric readouts visible to the naked eye or analyzed using software for quantitative measurements, that are a function of the concentration of the target molecule. Linkage specific glycolytic enzymes are used alongside glycosyl-dehydrogenase to generate a redox system capable of producing a colorimetric change in organic dyes. Heterologous expression of enzymes and immobilizing them on paper substrate increases the feasibility of the assay in terms of cost and durability. This paper based analytical device platform demonstrates its utility in qualitative and quantitative assays with potential for expansion to other molecules and applications.

Paper-based biosensors for glycan analysis

2019 Spring National Meeting

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