A component to understanding biological processes involves identifying the proteins expressed in cells as well as their modifications and the dynamics of processes. Several major technologies, but especially mass spectrometry, have benefited from large-scale genome sequencing of organisms. The sequence data produced by these efforts can be used to interpret mass spectrometry data of proteins and thus enables rapid and large-scale analysis of protein data from experiments. Advances in multi-dimensional separations as well as mass spectrometry have improved the scale of experiments for protein identification. This has improved the analysis of protein complexes, and more complicated protein mixtures. Quantitative mass spectrometry can be used to study biological processes such as protein-protein interactions, development or the effects of gene mutations on pathways. Recent studies on the loss of function mutant form of the Cystic Fibrosis Transport Regulator (DF508) as it progresses through the folding pathway will be presented. Through the study of protein-protein interactions and modifications that regulate maturation of CFTR, we now have a better understanding of the underlying biochemical process leading to loss of function.<sup>1,2</sup>

Understanding the molecular defect in Cystic Fibrosis

2019 Spring National Meeting

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