Mung bean (Vigna radiate) is an annual plant in the legume family and is an excellent source of protein, mineral and fiber, consuming as a whole snack, bean sprouts or bean noodles. This study investigated the residual characteristics of the fungicide hexaconazole in grain of mung bean after spraying hexaconazole 2 % suspension concentrate (SC) formulation. The residue of pesticide was analyzed by GC-MS/MS (Shimadzu GCMS-TQ8040) equipped with SGE-BPX5 column (0.25 mm × 30 m, 0.25 μm ). Method limit of quantitation (MLOQ) were 0.01 mg/kg. For the recovery test, 5 g of macerated grain of mung bean was treated with standard solutions at 10MLOQ and 50MLOQ levels, soaked with 10 mL of distilled water for 30 min and extracted with 10 mL of acetonitrile by shaking (30 min). Then 4 g of MgSO4 and 1 g of NaCl were added, shaken for 1 min and centrifuged at 3,500 rpm for 5 min. The supernatant (1 mL) was purified with dispersive SPE (7.5 mg of GCB, 25 mg of PSA and 150 mg of MgSO4) by shaking for 1 min, and centrifuged at 13,000 rpm for 5 min before analysis. Recovery rates were in the range of 70-120% (RSD≤20%) at two spiked levels. For field trial, the pesticide was treated on 5 plots by different days before harvest (Plot 1: 60/50/40 days, Plot 2: 50/40/30 days, Plot 3: 40/30/21 days, Plot 4: 30/21/14 days, Plot 5: 21/14/7 days before harvest) by three replicates. After harvest, the grain sample were analyzed as for recovery test. The residues in all plots were lower than MLOQ indicating that contamination of residue does not likely to occur in mung bean due to the protection role of mung bean pods against pesticide.

Residual characteristics of fungicide hexaconazole in minor crop mung bean during cultivation

2019 Spring National Meeting

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