Proper gene expression is essential for the survival of every cell. Once thought to be a passive transporter of genetic information, RNA has recently emerged as a key player in nearly every pathway in the cell. A full description of its structure is critical to understanding RNA function. Decades of research have focused on utilizing chemical tools to interrogate the structures of RNAs, with recent focus shifting to performing experiments inside living cells. I will detail the design and utility of chemical reagents used in RNA structure probing. I will also outline how these reagents have been used to gain a deeper understanding of RNA structure in cells and the<i> </i>merger of chemical probing with deep sequencing. Finally, I will outline some of the hurdles that remain in fully characterizing the structure of RNA inside living cells, and how chemical biology can uniquely tackle such challenges.

Chemical approaches for analyzing RNA structure inside cells

2019 Spring National Meeting

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