Supported lipid bilayers (SLBs) form when a vesicle solution is flowed over a surface such as silicon dioxide. Previous research has shown that SLB formation occurs in two steps. In the first step there is adsorption of vesicles from solution to the surface. In the second step the adsorbed vesicles deform until they are eventually under enough strain that they rupture to form a continuous phospholipid bilayer on the surface. The exact mechanism by which vesicles adsorb and then rupture is not known, though we have developed a model based on Michaelis-Menten Langmuir kinetics (MML) to help better understand this process, and have fitted quartz crystal microbalance (QCM) data to the model to acquire quantiative information on the formation process, including rate constants for the adsorption and rupture processes. In order to increase confidence in our model and to reduce the number of freely varying parameters we have looked at the role of flow rate and lipid concentration on SLB formation so that we can carry out constrained fits between our experimental QCM data and theoretical model.

Understanding the role of flow rate and lipid concentration in the kinetics of supported lipid bilayer formation

2019 Spring National Meeting

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