During downstream process development material is often limited so high throughput screening methods are commonly used to evaluate chromatography resins and operating conditions with minimal material.  Recombinant proteins provide a greater purification challenge compared to monoclonal antibodies as three-column processes may not be sufficient to meet final purity specifications or the process steps may require extensive optimization.<br/><br/>Recent studies have shown activated carbon to selectively separate host cell proteins (HCP) from monoclonal antibody protein. Activated carbon is available in filtration devices by several vendors, which makes it easily implementable in manufacturing. The smallest scale commercial devices available for process development however make it difficult to evaluate, develop and optimize this technology as large quantities of material are required even for a single run.<br/><br/>In this work we assessed the feasibility of HTS for the evaluation of loose activated carbon as a tool for HCP removal in the downstream purification processes for recombinant proteins.  The slurry concentration of activated carbon from multiple manufacturers was evaluated to make it amenable to a HTS format similar to chromatography media. Results from this work were compared to dynamic binding conditions in the filter format and used to implement a workflow for other molecules.  Our work will show that activated carbon in HTS format can be implemented to reduce material demands and allow for process optimization of this adsorbent for purification purposes.

Using high throughput screening to enable the development of an activated carbon filtration step for removal of host cell proteins in downstream processes

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.