Diamondback moth, Plutella xylostella is the major pest of cabbage (Brassica oleracea var. capitata L.) in worldwide. One way to reduce the population of Plutella xylostella is by interfering the hormonal control of insect and reproduction (Juvenile hormone) which will give sublethal effect on embryogenesis, metamorphosis and adult formation of diamondback moth. Juvenile hormone (JH) analogs mimic the action of JHs in a number of physiological processes. Farnesol dehydrogenase is an intermediate enzyme in JH III biosynthesis pathway in an insect. Thus, inhibition by farnesol dehydrogenase will give effect on formation of JH III. A new approach in designing of biorational insecticide is computer-aided pesticide design (CAPD) including homology model, virtual screening, and molecular docking. From homology tools using SWISS-MODEL server, showed 1XHL.1A protein got the highest similarities of 40% with the primary sequence of farnesol dehydrogenase from Plutella xylostella with 2.4 Å resolution. The inhibitory potency of 200 different types of compounds, which bearing similar structure with farnesol were tested in silico. Several farnesyl derivatives showed a good binding affinity towards farnesol dehydrogenase. Therefore, CAMD help in prediction and designing a new compound as an enzyme inhibitor towards enzyme farnesol dehydrogenase by looking at the interaction of the inhibitor with the enzyme.

Designing of biorational insecticide against diamondback moth, Plutella xylostella infestation on cabbage (Brassica oleracea var. capitata L.) by computer-aided pesticide design approach

2019 Spring National Meeting

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