Artificial lipid membrane is widely used for studying cell membrane property, drug development, and biosensor. Supported lipid bilayers (SLBs) are a kind of artificial lipid membrane situated at solid-liquid interface. SLBs have been used to investigate the fundamental physicochemical properties and molecular distribution of lipid bilayers. As a bacterial model cell system, a reconstituted Escherichia coli (E. coli) cell membrane has been studied. Understanding the bacterial membrane system supports to reveal the mechanism of molecular recognition and material transportation during bacterial infection for medical technology. The E. coli lipid extract contains three main phospholipids, phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CA). In this study, we formed SLBs of the E. coli extracted lipid on mica and thermally oxidized SiO2/Si substrates, and investigated them with atomic force microscopy and fluorescence-microscope-based methods. We found substrate-dependent SLB formation, and two- and three-dimensional morphologies depending on Ca2+ concentration. We also formed SLBs of E. coli extracted PE, PG and CA. These three major phospholipids showed various SLB formation and morphologies depending on the substrates and Ca2+ concentration.

Supported lipid bilayers of Escherichia coli extracted lipids and their substrate and calcium dependence

2019 Spring National Meeting

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