In the bioprocessing industry, there is a drive toward processes that reduce cost of goods while maintaining product quality and leveraging existing manufacturing facilities.  One potential opportunity for significant process compression while driving down process economics is in the mAb polishing train, which typically contains a series of disconnected bind/elute and flow-through chromatography operations. A fully-connected flow-through mAb polishing process can offer significant advantages over a traditional polishing template.<br/><br/>Within the Biosolve process modeling tool, a new template was created that incorporated an all-flow-through polishing train. Leveraging experimental data to populate the Biosolve models, results comparing the new flow-through polishing process to a traditional process template highlight significant benefits with the integrated flow-through solution – 40% cost of goods reduction, 80% reduction in buffer volume usage, 70% reduction in polishing process time, and 70% reduction in footprint. A sensitivity analysis highlights that these benefits are essentially independent of process scale, and are enhanced as bioreactor titer increases.<br/>

Using process modeling to quantify the benefits of an integrated mAb flow-through polishing solution

2019 Spring National Meeting

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