Biotin exists widely in food, but in small amount. In this study, HPLC analysis coupled with immunoaffinity column cleanup extaction was validated and applied for determination of total biotin content in four types of foods with different matrices (mandarin, corn, beef, and chestnut). The linearity of biotin standard was over R<sup>2</sup> = 0.999 in range of 0.015~0.960 μg/mL. Limits of detection and quantification of biotin were 0.007 and 0.023 μg/100 g, respectively. And the method showed good accuracy (99.28% recovery) from the certified reference material, SRM 1849a (Infant/adult nutritional formula). Precision of biotin was acceptable based on AOAC guidelines (3.4%). Biotin contents of the four types of foods were analyzed by using the validated immunoaffinity column-HPLC analysis. In the results, biotin contents of mandarin, corn, beef, and chestnut were 0.248 μg/100 g, 0.990 μg/100 g, 0.528 μg/100 g, 2.157 μg/100 g, respectively. The results of this study suggest that immunoaffinity column/HPLC assay would be effective to produce acceptable selectivity, sensitivity, accuracy, and precision of biotin analysis. Also, these results indicate that immunoaffinity column cleanup extaction is applicable for the determination of biotin at low levels in foods.

Method validation of immunoaffinity column-HPLC analysis for biotin in foods

2019 Spring National Meeting

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