Super-resolution fluorescence microscopy methods have made great strides over the past 10-15 years, but the high complexity and cost of many of these methods has hindered their dissemination. My group has recently developed new variations of the comparably simple and inexpensive super-resolution fluorescence microscopy method called expansion microscopy, which uses swellable hydrogels to physically enlarge fixed biological specimens so that features closer than the diffraction limit of light (~250 nm) are resolvable in the expanded specimen (~65 nm resolution). I will present new labeling methods we have developed for expansion microscopy and their application to diverse biological specimens including cultured cells, tissues, and clinical specimens.

Super-resolution microscopy made simple

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.