One of the many advantages of using cellulose nanocrystals (CNC) as an advanced material is that their organic chemistry enables facile surface functionalization. In this research, APTES-GA chemistry was used to attach antibodies to CNC. The resulting material was used to detect antibody-antigen binding of model cancer biomarkers on CNC films. Thermal gravimetric analysis coupled with Fourier transform infrared spectroscopy (TGA-FTIR) and fluorescence microscopy were used to evaluate initial functionalization, antibody immobilization, and antigen detection. In addition, both antibody immobilization and antigen detection were followed in real time using Quartz Crystal Microbalance with Dissipation monitoring (QCM-D).  In one scheme, the surface functionalization was performed on dispersed CNC. This enabled greater access to CNC’s high specific surface area.  In another scheme, the functionalization was performed on shear cast films. The effects of these two schemes on film microstructure and sensor performance were compared leading to new insights into the challenges and benefits of different approaches for fabricating CNC sensors.<br/>

Preparation of cellulose nanocrystal sensors for antibody-antigen binding of model cancer biomarkers

2019 Spring National Meeting

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