Single base DNA lesions are known to correlate with cancer through mutagenesis if the lesions are missed in the process of base excision repair (or BER). DNA glycosylases play a key role in recognizing and excising these DNA lesions to prevent such mutations. Uracil can be present in DNA through the deamination of cytosine, which can cause a mutagenic mispair of U:G. Uracil can also be mismatched with adenine. In this project, one base in the Dickerson-Drew Dodecamer (DDD) has been replaced with uracil, and the U:G and U:A mismatches are being studied. The different base-pairing and sequence contexts for uracil create different chemical shifts that have been studied using 2D solution NMR techniques to study proton interactions as well as 1D temperature effects of 31P. The data compares the changes in NOE intensity peaks in the modified sequences. The results indicate significant differences between the uracil-containing sequences and the control DDD sequence.

Effect of uracil in different sequence contexts on 2D NMR properties in DNA

2019 Spring National Meeting

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