The effects of the dihydrouracil lesion in DNA were studied using two dimensional NMR spectroscopy. The sequence used was based off of the Drew-Dickerson Dodecamer, with the cytosine in the three position replaced by a dihydrouracil. All of the nonexchangeable proton chemical shifts, with the exception of the H2, H5’, and H5’’, of the lesioned DNA were identified using NOESY spectra and then compared to the chemical shift values of the Drew Dickerson Dodecamer. The largest differences in chemical shifts were observed in the guanine 2, the nucleotide that is directly adjacent to the lesion in the 5’ direction. There were also significant changes in chemical shift in the guanine 10 and cytosine 11 residues, the base pairs of the lesion and guanine 2, respectively. Temperature dependent effects were also studied; the chemical shifts of the lesioned DNA segment were compared between 10 and 25C. The guanine 2 and the dihydrouracil 3 residues showed the greatest change in chemical shift between temperatures. Future work will include study of the imino exchangeable protons and the phosphorus backbone of the DNA segment, as well as the study of other DNA segments with the same lesion.

Study of the effects of dihydrouracil lesion in DNA on non-exchangeable chemical shifts and NOE intensities using two dimensional NMR spectrsocopy

2019 Spring National Meeting

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