Antibody recognition of carbohydrates plays a critical role in host immune defense. In addition, monoclonal antibodies that bind carbohydrates are valuable research tools and have numerous applications as diagnostics and therapeutics.  At present, the factors that influence antibody-carbohydrate interactions are not fully understood.  Our group has developed a carbohydrate antigen microarray as a high-throughput tool to study antibody affinity and selectivity.  The microarray contains a diverse collection of O-linked glycans, N-linked glycans, glycosaminoglycans, lipid glycans, glycopeptides, and glycoproteins.  We use our glycan microarray to study the effects of glycan structure, density, and presentation on antibody recognition.  In addition, we are using our glycan microarray to understand how the immune system evolves antibodies with high affinity and selectivity.  We compare binding properties of the initially formed germline antibodies to the affinity matured antibodies that arise after somatic hypermutation.  Collectively, these efforts are providing a better understanding of how antibodies recognize glycans as well as new insights for developing monoclonal antibodies to carbohydrates.<br/>

Investigating antibody-carbohydrate recognition with the aid of carbohydrate microarrays

2019 Spring National Meeting

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