Coactivator proteins are fundamental in the process of gene activation, forming protein-protein interactions (PPIs) with DNA-bound transcriptional activators. Thus, elucidating the structural and functional roles of coactivator-activator PPIs is key to understand gene expression. However, these interactions are particularly challenging to study due to their dynamic nature as well as their large, poorly-defined surface area. Furthermore, there features make rapid or high throughput analysis difficult. Presented here is the development of a colorimetric sensor array (CSA) for the detection and identification of coactivator proteins. This approach allows for a unique colorimetric fingerprint of proteins to be obtained based on interactions with a variety of sensors. To develop the array, a library of commercially-available dyes has been screened to construct a panel of molecules that exhibit unique spectroscopic profiles when exposed to two different coactivator domains, Med25-AcID and CBP-KIX. This array-based methodology will ultimately paint a more descriptive picture of the diverse structures the coactivators assume upon interacting with various transcriptional activators and small molecules.

Colorimetric sensor array for the characterization of the transcriptional coactivators Med25-AcID and CBP-KIX

2019 Spring National Meeting

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