Cell separation has been widely recognized as an essential technique in the regenerative medicine. Especially, non-label cell separation methods are required because they can realize simple separation steps with maintaining cellular activity. Among them, fibrous poly(N-isopropylacrylamide) (PNIPAAm), is a good candidate for realizing non-label cell separation. Fibrous PNIPAAm has a phase temperature close to the human body temperature, and shows wettability changes according to temperature change, which leads function of cell adsorption and desorption control. In order to realize sharp and efficient cell separation using fibrous PNIPAAm, quantitative analysis of protein / cell adsorption and desorption is necessary. In this study, we fabricated smart surface with fibrous PNIPAAm on QCM sensors by electrospinning method, and the adsorption and desorption behavior of fibronectin (FN), which is one of the proteins of the extracellular matrix, were directly measured by fabricated QCM sensor. From the frequency change of the QCM sensor in the solution containing FN (Figure 1), FN adsorbed on the surface of QCM sensor above the LCST of PNIPAAm, and FN desorbed from QCM below the LCST of PNIPAAm. It would be attributed to the temperature-dependent wettability change of PNIPAAm on the surface of QCM. This system was revealed to be effective for quantitative analysis of temperature-responsive protein behaviors on fibrous PNIPAAm

In situ monitoring of proteins adsorption and desorption on smart surface using quartz crystal microbalance

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.