Aptamers are short single-strand nucleic acids which can be isolated from a large combinatorial nucleic acid library, binding to the given targets with high affinity and specificity, by a process known as systematic evolution of ligands by exponential enrichment (SELEX). Because of several advantages of SELEX using live cells, or Cell-SELEX, varieties of live cells have been chosen as targets. For the live cells, various aptamers have been generated for a significant number of different cell membrane receptors including glucagon and protein tyrosine kinase-7 (PTK7). Here, we have successfully screened out new aptamer candidates for a cell membrane receptor expressed on Human Embryonic Kidney (HEK) cells transiently. Once successfully characterized by using different analytical methods, including surface plasmon resonance (SPR), flow cytometry, confocal microscopy, and so on, they will be used to identify the molecules inhibiting the receptor binding for pharmaceutical usages.<br/>

Screening of aptamers binding to a cell membrane receptor transiently expressed on the surface of the human cells

2019 Spring National Meeting

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