Oxidative DNA damage is a cause for cell death, cancer, and diseases. DNA damage is usually tracked at the cellular level by monitoring reactive oxygen species (ROS). Moving to the single DNA level often gives new insights into reactions that are missed in ensemble studies. Thus, it is advantageous to move ROS monitoring to the single molecule level. In this study we track the ROS produced by POPO-1, an intercalating DNA stain which causes photocleavage of DNA, through activation of CellROX Green. CellROX Green is a commercially available fluorescent DNA stain which is only fluorescent after reacting with superoxide or hydroxyl radicles. This allows us to track ROS production from POPO-1 in real time. This technique should be extendable to track ROS production from other sources at the single DNA level.

Monitoring reactive oxygen species production at the single DNA level

2019 Spring National Meeting

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