Signal amplification allows for sensitive and reliable detection of low concentrations of analyte. Polymerization amplification is a relatively new approach that takes advantage of the growth of a long polymer chain from one initiation event. The Cooley Lab aims to develop and evaluate a detection assay using a new signal amplification strategy that enables simple, real-time monitoring of an aqueous, fluorogenic polymerization. Controlled radical polymerization mechanisms were selected as the reactions are robust and sensitive to very low radical concentrations, can be compatible with enzymatic degassing techniques and have living character, which should allow for the amount of analyte to track with the expressed fluorescence. The development and optimization of these controlled radical fluorogenic polymerizations for use as a detection strategy will be presented.

Fluorogenic polymerization in aqueous media as a detection strategy

2019 Spring National Meeting

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