The miniaturization of analytical methods through microfabrication techniques allows for reduced sample size and analysis cost. Integrating multiple laboratory functions on a single device will ultimately allow the lab to be brought to the sample, circumventing the need for conventional methodology. The goal of this project is to investigate the use of segmented droplet microfluidic techniques to achieve these benefits for targeted chemical assays and for improved quantitative methodology. Forming segmented flow droplets is an essential step in conducting these analyses and the data collected suggests that multiple methods can be used to both effectively generate and detect droplets. An immiscible dual-phase system of an aqueous solution and perfluorodecalin oil was used in conjunction with a T-junction to form segmented flow droplets. Infusion and withdrawing techniques for droplet formation were studied using a syringe pump and vacuum-driven flow. Fluorescence measurements using an epifluorescence microscope followed by data analysis via ImageJ software gave high quality results for tracking light intensity levels in droplets of rhodamine B at a wide range of concentration levels. To generate microfluidic devices for droplet formation, a digital light projection (DLP) printer was used to generate molds that could be utilized for the formation of polydimethylsiloxane (PDMS) chips. Implementing these methods of droplet formation and detection will allow for low-cost, sensitive bioassays for medical diagnosis.

Segmented flow droplet formation using low-cost microfabrication techniques

2019 Spring National Meeting

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