Silica remains the most commonly used support material for the stationary phase in liquid chromatography (LC) due to its thermal stability, lack of shrinking or swelling when exposed to organic solvents, and separation reproducibility. The use of LC is limited to the advancements in the development of chromatographic column technology. Recently, the development of fibrous silica particles (FSPs) was achieved via sol-gel methods. FSPs posses an inherent fibrous morphology that provides very high surface area. Particles with high surface area are advantageous for LC because they can afford higher loading capacity than conventional regular particles. FSPs were derivatized with n-octyldimethoxymethyl silane (C8-DMS) to evaluate their chromatographic performance under reversed phase conditions. The particles were examined via FTIR spectroscopy, gas adsorption analysis, thermogravimetric analysis, and scanning electron microscopy before and after functionalization with C8-DMS. Columns were packed with the functionalized FSPs and their chromatographic performance was evaluated using high performance liquid chromatography (HPLC). Herein, we present the physicochemical characterization of the C8-DMS functionalized particles and their preliminary evaluation under HPLC conditions. The chromatographic performance of columns packed with FSPs is also compared with columns packed with conventional packing material.

Evaluation of fibrous silica particles for liquid chromatography

2019 Spring National Meeting

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