While most of disposable bioreactors use a cylindrical design, the cubical biocontainer design allows for a wide range of agitation and air flow to be achieved. Computational fluid dynamics (CFD) modeling shows excellent mixing performance that matches the typical cylindrical format of stainless steel bioreactors. To demonstrate the performance scalability of novel square geometry bioreactors, we adopted a strategy by maintaining constant power input per volume and superficial gas velocity for fed-batch culture of a CHO-S cell line expressing IgG1 monoclonal antibody. From our case studies, similar growth profile, cell density, cell viability, nutrients/metabolites profile, mAb titer and quality were evident. The results illustrate scalable performance from benchtop cylindrical 1.4 and 10 L bioreactors to 50, 200, 1000, and 2000 L cubical bioreactors.

Scalability of novel geometry single-use bioreactors

2019 Spring National Meeting

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