Diacyglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), is an uncommon membrane lipid found in plants, yeast, and other microorganisms but never in mammals. DGPP is formed from PA, which, in plants, is formed as a response to stressful environmental conditions such as extreme temperature, salinity, and pathogen attack. The function of the formation of DGPP during stress is unclear; however, DGPP is unique as it is one of few membrane lipids with a pyrophosphate in the head group. This pyrophosphate head group is likely crucial to the function of DGPP. Furthermore, genetic approaches have thus far failed to elucidate a function for DGPP, other than verify its involvement in stress signaling. Therefore, the physical-chemical and protein-lipid interaction properties were analyzed to elucidate the function of DGPP. We focus on the ionization of the pyrophosphate head group in model membrane systems of increasing complexity. Specifically, we evaluate the ionization of DGPP when present together with PA in model systems of phosphatidylcholine or phosphatidylcholine plus phosphatidylethanolamine. Additionally we evaluate the binding of PA binding proteins to DGPP. The results shed light on the function of the enigmatic stress signaling lipid DGPP.<br/>

Dynamic interplay between PA and DGPP regulates lipid negative charge and protein-lipid interactions

2019 Spring National Meeting

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