Vaccine for smallpox was a breakthrough invention by Edward Jenner and since 18th century saved millions of lives. From that time many other vaccines were developed extending the human lifetime, quality and preventing worldwide epidemies. Though vaccination is a very attractive way of prevention against diseases, it’s discovery and production is very challenging. Especially the lack of templated approaches increases the costs and timelines required. Today we would like to address the potential simplification of vaccine manufacturing, while introducing the first steps towards templated processing. New technologies, as custom affinity chromatography, could enable purification of vaccines with high specificity and elevated process recovery rates. Moreover, the level of impurities with similar physical properties to the target molecule (e.g. DNA and scaffolds thereof, HCP) can be reduced. Ironically the challenge of custom affinity chromatography is the same as for the vaccines: discovery and the development time. To address major challenges, our approach is based on a templated way of custom affinity chromatography development paradigm: a) ligand discovery process, based on the existing ligand libraries; b) E.Coli expression of identified tagged ligands; C) Epoxy based coupling for orientated immobilization; D) small scale manufacturing including product quality control. The wide selection of different supports and chemistries is essential in enabling fast development and the choice of best strategies. We believe, that custom affinity chromatography is just one, but essential peace to assure faster and more reliable vaccine manufacturing process in future reducing the manufacturing steps and simplifying the whole process.

Custom affinity chromatography for vaccine purification: A new PD paradigm

2019 Spring National Meeting

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