Glioblastoma multiforme (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs). GSCs reside near perivascular niche and hypoxic region where they can avoid chemotherapy and immune surveillance. Thus, there is a great need to develop a proper platform for the study of GBM and their therapy. Current GSC expansion methods rely on growing them in serum-free media, resulting in tumor sphere formation. This conventional method, however, often results in large cell aggregation instead of forming sphere. It is also difficult to control the size of the spheres. Many prior studies focused on modeling GBM using hydrogels, but these methods are limited to small scale with complex procedures. Here, we suggest a simple and more in vivo-like GBM culture method by optimizing the shear stress in a small scale bioreactor. We cultured patient-derived GBM cell lines with controlled size of GBM tumorsphere. GSCs grown under shear stress showed increase of Notch signals as well as pericytes markers which indicate characteristics of GSCs in perivascular niche. Further, GSCs grown under shear stress showed increase of CD133 population. Collectively, GSC culture under optimized shear stress can model GSCs in perivascular niche including their microenvironment and GSCs expansion.

Expansion of NOTCH<sup>high</sup> and CD133<sup>high</sup> GSC-enriched niche model using a small scale bioreactor

2019 Spring National Meeting

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Expansion of NOTCHhigh and CD133high GSC-enriched niche model using a small scale bioreactor

Expansion of NOTCH^high and CD133^high GSC-enriched niche model using a small scale bioreactor