Optimal protein-polymer conjugates should have intact biological function of the protein combined with the processability of the polymer. With this work, we aim to capture these qualities and generate solution-stable protein-polymer conjugates using the recombinantly expressed human carbohydrate-binding protein, galectin-3 (gal3). Conjugates are synthesized using both full gal3 and a truncated sugar-binding domain-only construct. Cysteines substituted into gal3 using site-directed mutagenesis will be the reactive handles for grafting-to polymer conjugations. Proteins will initially be conjugated with polyethylene glycol (PEG) as a model polymer to test for solution stability and ligand-binding ability. These will serve as preliminary studies for future applications of surface-immobilized gal3-polymer conjugates.

Generation of solution-stable galectin-3 polymer conjugates

2019 Spring National Meeting

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