The most common treatment of hepatocellular carcinoma is chemotherapy via transarterial chemoembolization (TACE) using doxorubicin (DOX) chemotherapeutic agent. DOX preferentially binds to guanine-cytosine sites in DNA and inhibits replication. TACE allows a significant amount of DOX dosage to be administered directly into the tumor to reduce its size by destroying the rapidly dividing cells, however, the remaining drug enters systemic circulation where it also destroys healthy cells. To prevent DOX systemic circulation and reduce side effects, this research seeks to develop a filter device that will be deployed downstream from the tumor to capture residual DOX, isolating its presence in the hepatic tumor. Cotton fabric was used as a substrate to assess DOX sequestration capability then coated with functionalized with silanes which served as DNA linking molecules. 3-glycidoxylpropyltrimethoxy silane (GDPTMS), 3-isocyanotopropyltrimethoxy silane (ICPTMS), and 3-aminopropyltriethoxy silane (APTES) were applied to the surface via chemical vapor deposition (CVD) and via reaction in toluene. Herring DNA was reacted with surface functional groups and the reaction confirmed via Energy-dispersive spectroscopy (EDS). The functionalized cotton was then immersed in DOX solution at physiological temperature to assess capture capability with increasing time. Fluorescence spectroscopy determined the ICPTMS-coated sample showed the most DOX capture after 1 minute (17%) and after 10 minutes, both ICPTMS and APTES-coated samples captured 66% of DOX present in solution. The capability of the functionalized cotton surfaces to sequester DOX in complex media will be assessed in phosphate buffer solution (PBS) and blood serum. Additionally, various silanes will be used to enhance reactivity with DNA and increase DOX capture.

Functionalization of cotton fabric substrate for enhanced sequestration of Doxorubicin (DOX) chemotherapeutic agent

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.