Multimodal membranes are a novel class of chromatography media that bind proteins and bioprocess impurities through combinations of Coulombic interactions, hydrophobic interactions and hydrogen bonding. Because of the multiple modes of binding, these membranes can operate over a wider range of conditions than conventional membranes, enabling their use in chromatographic purification steps with minimal or no pretreatment of the feed.<br/>In this study, we are evaluating the performance of Purilogics’ multimodal anion exchange membrane in bind-and-elute mode under a variety of industrial relevant conditions. In this presentation, we will present performance data from dynamic binding capacity (DBC) measurements for bovine serum albumin (BSA), human immunoglobulin G (hIgG), and salmon sperm DNA (ssDNA) for a range of conductivities (0 -30 mS/cm) and pH values (5-9). We will show that these novel membranes have high capacities at short residence times even under conditions of high ionic conductivity. Dynamic binding capacities for BSA, IgG and ssDNA varied with pH and ionic conductivity. The isoelectric point of the protein determined the trend of DBC with pH. DBC also varied with ionic conductivity, often showing a maximum at 5.7 mS/cm (50 mM NaCl). Based on comparisons to commercial membrane products, these high-capacity membranes can be an alternative for purifying large bioprocess volumes using short residence times without dilution or desalting of the feed.<br/>

High-capacity multimodal anion-exchange membranes for purification of biologics

2019 Spring National Meeting

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