Protein A chromatography is a platform technology for monoclonal antibody (mAb) capture step purification in the industry due to its high selectivity. However, it is not uncommon to spend from hours to a full day to complete one purification cycle using existing Protein A resin columns (not counting the significant time spent on column packing). Capture purification has thus become a critical bottleneck during downstream purification.<br/><br/>To address this challenge, Purilogics developed a novel Protein A membrane adsorber and demonstrated that the membrane provides high binding capacity at exceedingly short residence time for a variety of antibodies. The membrane was validated through purification of a portfolio of mAb supernatants. The purified antibody streams are characterized by high recovery and low levels of host cell proteins and DNA. Lastly, we will present data from a bind-and-elute cycling study to illustrate reusability of the new membrane.<br/>

New protein A membranes for the rapid isolation and purification of monoclonal antibodies

2019 Spring National Meeting

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