Hypercholesterolemia is considered a major risk factor of cardiovascular disease (CVD), the potential for lactic acid bacteria (LAB) to lower serum cholesterol has reported before. Therefore this study aimed at identity LAB strains of with optimal cholesterol lowering ability and probiotic properties. A total of 75 LAB strains from traditional fermented pickles and infant feces were investigated on cholesterol-lowering ability by gas chromatography method, and Lactobacillus acidophilus ATCC 43121 used as the control strain. Lactobacillus plantarum E680, with high cholesterol-lowering ability and good probiotic properties in vitro, was selected for further study. Results showed that L. plantarum E680 reduced the total cholesterol level by 66.84 % in broth culture, which was significantly higher (P&lt;0.05) than that of ATCC 43121 (58.31 %); showed the highest acid and bile tolerance (tolerance rate were all over 80 %); was sensitive to five of eight antibiotics tested (ampicillin, cefazolin, erythromycin, sulfamethoxazole, and chloramphenicol); inhibited the growth of four pathogenic bacteria, with Staphylococcus aureus being most sensitive, and Salmonella typhimurium the least sensitive. Animal model was also used to test the efficacy of strain. Mice were randomly divided in control group (gavage of sterile saline), model group (gavage of high-fat emulsion), and E680 group (gavage of high-fat emulsion plus E680 (109 CFU/mouse/d)). In vivo testing found that L. plantarum E680 suppressed body weight gain of mice without effect the food intake; prevented elevation of the total cholesterol and low-density lipoprotein cholesterol levels, which were 10.71 % and 16.47 % lower than levels observed in model mice; was no effect on high-density lipoprotein cholesterol and triglyceride levels. In conclusion, L. plantarum E680 has optimal cholesterol lowering ability and probiotic properties, which have potential to development functional food for improving human health.

Probiotic characteristics of Lactobacillus plantarum E680 and its effect on hypercholesterolemic mice

2019 Fall National Meeting

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