The protective effect of wheat alkylresorcinols (ARs) on ARPE-19 cells against oxidative stress and the possible underlying mechanism were investigated in this study. The results showed that ARs significantly inhibited 300 μM H2O2-induced ARPE-19 cells damage and reactive oxygen species (ROS) generation by 19 % and 32 %, respectively. Moreover, ARs treatment increased NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increasing transcription of anti-oxidant responsive, GCL, NQO1 and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated ARs-mediated HO-1 transcription, and almost abolished ARs-mediated cytoprotection against H2O2 induced cell damage. Further studies showed that the protective effect of ARs was depended on Akt activation. Taken together, these results demonstrated that ARs could protect ARPE-19 cells from oxidative stress induced cell damage possibly through Akt dependent Nrf2/HO-1 signaling.

Protective effect of wheat alkylresorcinols against hydrogen peroxide-induced oxidative stress in ARPE-19 cells

2019 Fall National Meeting

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