Mass spectrometry-based quantitation method has advanced rapidly. General methods rely on the use of authentic target compounds or isotope-labeled compounds as standards, which might be unavailable or difficult to synthesize. To tackle this challenge, a novel approach for absolute quantitation without the use of standards is presented based on electrochemistry (EC) combined with mass spectrometry (MS). In this approach, a target compound is allowed to undergo electrochemical oxidation and then analyzed by MS. The oxidation current from EC measurement provides information about the amount of oxidized analyte, based on the Faraday’s Law. Meanwhile, the oxidation yield can be determined from analyte MS signal changes upon electrolysis. Therefore, total amount of analyte can be determined. In combination with liquid chromatography (LC), the method can be applicable to mixture analysis. The method is applicable to electroactive organic compounds, peptides and proteins.

Electrochemistry-assisted absolute quantitation by mass spectrometry without the use of standards

2019 Fall National Meeting

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